Data for the Constructed Mutant: YCNEdd

General Information

Name of the MutantYCNEdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size285 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:
Correspondence:


Mutant Construction for YCNEdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctcgag tag) ycnE-F-F(Xho) :ctcctcgagGCCTGCTCTCATTATCGGC
Reverse Primer:(ggaggatcc tag) ycnE-F-R(Bam):ggaggatccCGATCATGTAAATCTCCTCCG
Downstream:
Forward Primer:(aagaagctt tag) ycnE-B-F(Hin):aagaagcttAGCGAGTAAAGGAAGGG
Reverse Primer:(ctcctcgag tag) ycnE-B-R(Xho): ctcctcgagCTGAACGGTCGGCAGGATGCC
Linearization:
Length of the Deletion: 8 to 279 Length: 272


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YCNEdd