Data for the Constructed Mutant: YCLQd

General Information

Name of the MutantYCLQd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size951 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCLQd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yclQ1: gcgcaagcttTGATGATATAGTAGCGGGCC
Reverse Primer:(gccgagatct tag) yclQ2: gccgagatctAGTGATCAATGTTTGCGAGC
Length of the Cloned Region: 219 to 535 Length: 317
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCLQd