Data for the Constructed Mutant: YCLPd

General Information

Name of the MutantYCLPd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size756 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCLPd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yclP1: gcgcaagcttAGCAAACAATATGGCGGGAA
Reverse Primer:(gccgagatct tag) yclP2: gccgagatctACCCAGTCTTCCTCTGTCAG
Length of the Cloned Region: 22 to 341 Length: 320
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCLPd