Data for the Constructed Mutant: YCLMd

General Information

Name of the MutantYCLMd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1362 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCLMd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yclM1: gcgcaagcttTTAAATTCGGAGGCAGCTCA
Reverse Primer:(gccgagatct tag) yclM2: gccgagatctTTGGCATTGTTATCCTCTCC
Length of the Cloned Region: 11 to 374 Length: 364
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCLMd