Data for the Constructed Mutant: YCLJd

General Information

Name of the MutantYCLJd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size681 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCLJd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yclJ1: gcgcaagcttTAGTGTATGTACGATGACGG
Reverse Primer:(gccgagatct tag) yclJ2: gccgagatctCTGTCGCCTTATATCTTCTG
Length of the Cloned Region: 30 to 364 Length: 335
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCLJd