Data for the Constructed Mutant: YCLEd

General Information

Name of the MutantYCLEd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size843 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCLEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yclE1: gcgcaagcttTCACTCGGACGGCTTTGATT
Reverse Primer:(gccgagatct tag) yclE2: gccgagatctTCCTGGCATACTCTAAAGCC
Length of the Cloned Region: 21 to 319 Length: 299
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCLEd