Data for the Constructed Mutant: YCLBd

General Information

Name of the MutantYCLBd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size612 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCLBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yclB1: gcgcaagcttTAAAGGAGGGGGCAAAGTGA
Reverse Primer:(gccgagatct tag) yclB2: gccgagatctCCATTCCTGTGCGAATGCTT
Length of the Cloned Region: 21 to 328 Length: 308
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCLBd