Data for the Constructed Mutant: YCICd

General Information

Name of the MutantYCICd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1191 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCICd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yciC1: gcgcaagcttGTGGAAATGTCAAATGGCTG
Reverse Primer:(gccgagatct tag) yciC2: gccgagatctTTTGATCGATCAAGAGGTCG
Length of the Cloned Region: 190 to 520 Length: 331
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCICd