Data for the Constructed Mutant: YCGSd

General Information

Name of the MutantYCGSd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size852 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCGSd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycgS1: gcgcaagcttCGGGCATTCAAATTGTTACG
Reverse Primer:(gccgagatct tag) ycgS2: gccgagatctGGAAGAGAATTTCCCCCAAT
Length of the Cloned Region: 93 to 424 Length: 332
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCGSd