Data for the Constructed Mutant: YCGKd

General Information

Name of the MutantYCGKd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size972 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCGKd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycgK1: gcgcaagcttGGCTTACCCTTTTTCACAGA
Reverse Primer:(gccgagatct tag) ycgK2: gccgagatctTGTAATGGAGAGCCGTTCAT
Length of the Cloned Region: 131 to 474 Length: 344
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCGKd