Data for the Constructed Mutant: LCTPd

General Information

Name of the MutantLCTPd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1623 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for LCTPd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycgC1: gcgcaagcttATTGGCTACATCGTCCTGAT
Reverse Primer:(gccgagatct tag) ycgC2: gccgagatctACAGCTCAAGAGCAGACAAA
Length of the Cloned Region: 232 to 568 Length: 337
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of LCTPd