Data for the Constructed Mutant: YCGAd

General Information

Name of the MutantYCGAd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1188 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YCGAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) ycgA1: gcgcaagcttAGCACCCATCATCTTTCTGA
Reverse Primer:(gccgagatct tag) ycgA1: gccgagatctATTCCTGAGAAAAGAGGGAG
Length of the Cloned Region: 12 to 371 Length: 360
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCGAd