Data for the Constructed Mutant: YCCHd

General Information

Name of the MutantYCCHd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size699 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCCHd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yccH-1: gcgcaagcttAGAGAAGTTAGAAGCGATCG
Reverse Primer:(gccgagatct tag) yccH-2: gccgagatctTATACGCCCATTCAAACTCG
Length of the Cloned Region: 42 to 387 Length: 346
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCCHd