Data for the Constructed Mutant: YCCGd

General Information

Name of the MutantYCCGd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BglII
Gene Size954 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for YCCGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yccG-1: gcgcaagcttATGTTATCAGGGAGCTGCTG
Reverse Primer:(gccgagatct tag) yccG-2: gccgagatctTGCTGTTCAGCTTGAGTCAG
Length of the Cloned Region: 48 to 362 Length: 315
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YCCGd