Data for the Constructed Mutant: HISTd

General Information

Name of the MutantHISTd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size741 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for HISTd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(ggaagc tag) hisT-F: ggaagcTTCCGGTCGTTTCGTCTGGA
Reverse Primer:(ttggatc tag) hisT-R: ttggatcCTTTTTTGACTGCGCTAAAT
Length of the Cloned Region: 131 to 328 Length: 198
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of HISTd