Data for the Constructed Mutant: RAPCd
General Information
Name of the Mutant
RAPCd (disruption)
Host
168
Used Vector
pMutin2
Used Enzyme Sites
HindIII and BamHI
Gene Size
1146 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
no
Mutant Submission to NIG:
yes
Correspondence:
Mutant Construction for RAPCd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
gcgcaagctt
tag)
yclL1:
gcgcaagcttGTAATTCCTTCTTCAGCGGT
Reverse Primer:
(
gccgagatct
tag)
yclL2:
gccgagatctCCTGGTTGCTGTCAATGTTT
Length of the Cloned Region: 13 to 280 Length: 268
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:yes
Enzyme: EcoRV
Probe used: pMutin2
Figure
Comment: ;.
1st Level Phenotype Analysis of
RAPCd