Data for the Constructed Mutant: RAPCd

General Information

Name of the MutantRAPCd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1146 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for RAPCd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gcgcaagctt tag) yclL1: gcgcaagcttGTAATTCCTTCTTCAGCGGT
Reverse Primer:(gccgagatct tag) yclL2: gccgagatctCCTGGTTGCTGTCAATGTTT
Length of the Cloned Region: 13 to 280 Length: 268
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of RAPCd