Data for the Constructed Mutant: MSMXd

General Information

Name of the MutantMSMXd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1095 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for MSMXd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) msmXH: GCCGaagcttTATGATCAGAAGGAACCGGC
Reverse Primer:(agatct tag) msmXBG: GCGCagatctAGCCCGAACGCGATATTATC
Length of the Cloned Region: 37 to 302 Length: 266
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of MSMXd