Data for the Constructed Mutant: YXERd

General Information

Name of the MutantYXERd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size1110 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXERd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxeRH: GCCGaagcttTTTACTATGGGGAGCAGCGG
Reverse Primer:(agatct tag) yxeRBG: GCGCagatctAAACACCCATGAAAACAGCC
Length of the Cloned Region: 42 to 333 Length: 292
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXERd