Data for the Constructed Mutant: YXEPd

General Information

Name of the MutantYXEPd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size1140 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXEPd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxePH: GCCGaagcttAATCAATATGCGGCGTGATC
Reverse Primer:(agatct tag) yxePBG: GCGCagatctATTCAGCAACATCGCTGTGC
Length of the Cloned Region: 30 to 345 Length: 316
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXEPd