Data for the Constructed Mutant: YXENd

General Information

Name of the MutantYXENd (disruption)
Host168
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size672 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YXENd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxeNH: GCCGaagcttTCATGATATCAGCGTTCCCG
Reverse Primer:(agatct tag) yxeNBG: GCGCagatctATGATGGCAGCTGTGAGAGC
Length of the Cloned Region: 23 to 290 Length: 268
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YXENd