Data for the Constructed Mutant: YXELd

General Information

Name of the MutantYXELd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size495 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXELd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxeLH: GCCGaagcttTGCAGTTGAACGTGATGCCG
Reverse Primer:(agatct tag) yxeLBG: GCGCagatctGTCTTCTTCCATGACGTAGC
Length of the Cloned Region: 21 to 177 Length: 157
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXELd