Data for the Constructed Mutant: YXELd
General Information
Name of the Mutant
YXELd (disruption)
Host
168
Used Vector
pMutin2
Used Enzyme Sites
HindIII and BamHI
Gene Size
495 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
yes
Mutant Submission to NIG:
yes
Correspondence:
Mutant Construction for YXELd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
aagctt
tag)
yxeLH:
GCCGaagcttTGCAGTTGAACGTGATGCCG
Reverse Primer:
(
agatct
tag)
yxeLBG:
GCGCagatctGTCTTCTTCCATGACGTAGC
Length of the Cloned Region: 21 to 177 Length: 157
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR: yes
Primer sets used for the check
No.
Primer set
Length of PCR product
1
Forward Primer
yxeLup
tgtaaaacgacggccagtTAGATGAAGCCACGATACCG
Reverse Primer
yxeLdn
caggaaacagctatgaccTCAAGGTGATGGTCGCGATG
Digestion of the PCR product: yes
Enzyme used: EcoRV
Figure
Comment: ;.
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
YXELd