Data for the Constructed Mutant: YXEId

General Information

Name of the MutantYXEId (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size984 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXEId (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxeIH: GCCGaagcttCAGAGGTGATTCTCTATCCG
Reverse Primer:(agatct tag) yxeIBG: GCGCagatctGACAATATCCAGTGAAGCGG
Length of the Cloned Region: 83 to 437 Length: 355
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXEId