Data for the Constructed Mutant: YXEGd

General Information

Name of the MutantYXEGd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size555 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXEGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxeGH: GCCGaagcttAAGACTGGTGGCAGCG
Reverse Primer:(agatct tag) yxeGBG: GCGCagatctAGTACATCATAGCGCGCATC
Length of the Cloned Region: 21 to 193 Length: 173
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXEGd