Data for the Constructed Mutant: YXEDd

General Information

Name of the MutantYXEDd (disruption)
Host168
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size351 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YXEDd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxeDH: GCCGaagcttTTGCAGCAGCTGAACCAATG
Reverse Primer:(agatct tag) yxeDBG: GCGCagatctTGTGCAGCTTGTTGTTCGTG
Length of the Cloned Region: 22 to 173 Length: 152
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YXEDd