Data for the Constructed Mutant: YXEBd

General Information

Name of the MutantYXEBd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size957 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXEBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxeBH: GCCGaagcttTGCTTGTCCTGCTTCTCATG
Reverse Primer:(agatct tag) yxeBBG: GCGCagatctTTAAGTCAGGCTGAAGCTCC
Length of the Cloned Region: 29 to 343 Length: 315
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXEBd