Data for the Constructed Mutant: YXEAd

General Information

Name of the MutantYXEAd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size345 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YXEAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) yxeAH: GCCGaagcttGCGGCTGCTGCCGTAATATG
Reverse Primer:(agatct tag) yxeABG: GCGCagatctTCCCGGATGCATTATATCCG
Length of the Cloned Region: 34 to 202 Length: 169
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YXEAd