Data for the Constructed Mutant: YFIHd

General Information

Name of the MutantYFIHd (disruption)
Host168
Used VectorpMutin2
Used Enzyme SitesEcoRI and BamHI
Gene Size939 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YFIHd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccggaattc tag) iHEF: gccggaattcTCTTATGTAACAGACAGCC
Reverse Primer:(gcgcggatcc tag) iHBR: gcgcggatccGCGCAAAGTGTCATGTTC
Length of the Cloned Region: 10 to 212 Length: 203
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YFIHd