Data for the Constructed Mutant: YFIGd

General Information

Name of the MutantYFIGd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1446 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFIGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) iGHF: gccgaagcttAAGCTTAGCTCATAAAGGG
Reverse Primer:(gcgcggatcc tag) iGBR: gcgcggatccAGACGTCCTCCAAACATC
Length of the Cloned Region: 39 to 248 Length: 210
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFIGd