Data for the Constructed Mutant: YFIEd

General Information

Name of the MutantYFIEd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size855 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFIEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) iEHF: gccgaagcttATGAGGATACACACATTGG
Reverse Primer:(gcgcggatcc tag) iEBR: gcgcggatccATGCCGTGTTCAATCAGG
Length of the Cloned Region: 14 to 293 Length: 280
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFIEd