Data for the Constructed Mutant: YFICd

General Information

Name of the MutantYFICd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1812 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFICd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) iCHF: gccgaagcttGCAAGCCTTTCCAATATCC
Reverse Primer:(gcgcggatcc tag) iCBR: gcgcggatccACGATAAAATGGTCGATGG
Length of the Cloned Region: 17 to 236 Length: 220
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFICd