Data for the Constructed Mutant: YFIBd

General Information

Name of the MutantYFIBd (disruption)
Used VectorpMutin2
Used Enzyme SitesSmaI and BamHI
Gene Size1719 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YFIBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccggggccc tag) iBHF: gccggggcccAACCATATTCATTGCTGGC
Reverse Primer:(gcgcggatcc tag) iBBR: gcgcggatccCTCGTATCATAGGAAAAGC
Length of the Cloned Region: 26 to 266 Length: 241
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YFIBd