Data for the Constructed Mutant: GLVCd

General Information

Name of the MutantGLVCd (disruption)
Used VectorpMutin2
Used Enzyme SitesHindIII and BamHI
Gene Size1581 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for GLVCd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gccgaagctt tag) glv-2HF: gccgaagcttTCGTCGGTATCAGCACG
Reverse Primer:(gcgcggatcc tag) glv-2BR: gcgcggatccGACCTCTTGATTCATGTCG
Length of the Cloned Region: 74 to 360 Length: 287
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of GLVCd