Data for the Constructed Mutant: GALEd

General Information

Name of the MutantGALEd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size1017 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for GALEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagctt tag) galEH: GCCGaagcttTACATTGGCAGCCACACATG
Reverse Primer:(agatct tag) galEBG: GCGCagatctTGACGCCGTATTTCTCCATG
Length of the Cloned Region: 31 to 349 Length: 319
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of GALEd