Data for the Constructed Mutant: YRKNd

General Information

Name of the MutantYRKNd (disruption)
Host
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size555 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YRKNd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:( tag) yrkN-F: TCGCCGTGATTGAAACG
Reverse Primer:( tag) yrkN-R: TGGCTGTGATATTCTGG
Length of the Cloned Region: -1000 to -1001 Length: 2
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YRKNd