Data for the Constructed Mutant: YQKLd

General Information

Name of the MutantYQKLd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size447 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YQKLd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yqkL-F: aagaagcttAACCGTATTGATCGTATTAAG
Reverse Primer:(taatacgactcactatagggcgaggatcc tag) yqkL-R: taatacgactcactatagggcgaggatccTGTAGCGAGACCGATCTC
Length of the Cloned Region: 7 to 174 Length: 168
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YQKLd