Data for the Constructed Mutant: YQKJd

General Information

Name of the MutantYQKJd (disruption)
Host
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1317 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no
Correspondence:


Mutant Construction for YQKJd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yqkJ-F: aagaagcttGCGAAACACATGATTCGG
Reverse Primer:(taatacgactcactatagggcgaggatcc tag) yqkJ-R: taatacgactcactatagggcgaggatccTGCCAGTGAACGGATCGG
Length of the Cloned Region: 7 to 318 Length: 312
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YQKJd