Data for the Constructed Mutant: YQKId

General Information

Name of the MutantYQKId (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1404 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YQKId (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yqkI-F: aagaagcttGATGTAAGATTGCCAACAC
Reverse Primer:(taatacgactcactatagggcgaggatcc tag) yqkI-R: taatacgactcactatagggcgaggatccGTGAATAAATTCCAGTCC
Length of the Cloned Region: 7 to 315 Length: 309
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YQKId