Data for the Constructed Mutant: YQKGd

General Information

Name of the MutantYQKGd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size555 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YQKGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yqkG-F: aagaagcttAAATCATTAGAAGAAAAAACAATTGCC
Reverse Primer:(taatacgactcactatagggcgaggatcc tag) yqkG-R: taatacgactcactatagggcgaggatccTTTCCCTTCATCTGTGAC
Length of the Cloned Region: 4 to 168 Length: 165
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YQKGd