Data for the Constructed Mutant: YQJZ

General Information

Name of the MutantYQJZ (disruption)
Host
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size342 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no
Correspondence:


Mutant Construction for YQJZ (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yqjZ-F: aagaagcttGATTTTTTATCAAAAACACCTG
Reverse Primer:(taatacgactcactatagggcgaggatcc tag) yqjZ-R: taatacgactcactatagggcgaggatccAGGCTGATCCGCCGCAAG
Length of the Cloned Region: 7 to 132 Length: 126
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YQJZ