Data for the Constructed Mutant: YQJYd

General Information

Name of the MutantYQJYd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size468 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YQJYd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yqjY-F: aagaagcttGATATTCGAACAATCACG
Reverse Primer:(taatacgactcactatagggcgaggatcc tag) yqjY-R: taatacgactcactatagggcgaggatccGTCCTGAAAATGCTCAAAAAATAG
Length of the Cloned Region: 4 to 123 Length: 120
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YQJYd