Data for the Constructed Mutant: YQJSd

General Information

Name of the MutantYQJSd (disruption)
Host
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size879 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no
Correspondence:


Mutant Construction for YQJSd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yqjS-F: aagaagcttTCGATTGCTGTATCTGAAG
Reverse Primer:(taatacgactcactatagggcgaggatcc tag) yqjS-R: taatacgactcactatagggcgaggatccCAAGATCCGCGCCGTCGTGC
Length of the Cloned Region: 4 to 249 Length: 246
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YQJSd