Data for the Constructed Mutant: YQIVd

General Information

Name of the MutantYQIVd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1371 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YQIVd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yqiV-F: aagaagcttACTGAGTATGACGTAGTC
Reverse Primer:(taatacgactcactatagggcgaggatcc tag) yqiV-R: taatacgactcactatagggcgaggatccATCAACAACGGCTTGCTTACG
Length of the Cloned Region: 7 to 276 Length: 270
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YQIVd