Data for the Constructed Mutant: YQHYd

General Information

Name of the MutantYQHYd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size405 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YQHYd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yqhY-F: aagaagcttGACAACAGCTTGCTTAAAATGG
Reverse Primer:(taatacgactcactatagggcgaggatcc tag) yqhY-R: taatacgactcactatagggcgaggatccTCCGCGCATTTCGGGAAC
Length of the Cloned Region: 7 to 135 Length: 129
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YQHYd