Data for the Constructed Mutant: COTNd

General Information

Name of the MutantCOTNd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size783 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for COTNd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:( tag) cotN-F: GCTTCTGCAGCACTAGG
Reverse Primer:( tag) cotN-R: CTTCAAACTGGCTGAGG
Length of the Cloned Region: -1000 to -1001 Length: 2
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of COTNd