Data for the Constructed Mutant: YBBAd

General Information

Name of the MutantYBBAd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size567 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:

Mutant Construction for YBBAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(ggaa tag) ybbA-F: ggaaGCTTGATGAATACACCCCAT
Reverse Primer:(ttgga tag) ybbA-R: ttggaTCCCGATAGACATGAAAACA
Length of the Cloned Region: 30 to 361 Length: 332
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YBBAd