Data for the Constructed Mutant: YBXHdd

General Information

Name of the MutantYBXHdd (deletion (double-cross))
Host168
Used VectorpMutin1
Used Enzyme SitesHindIII;,BamHI;,XhoI
Gene Size189 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YBXHdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(gtctcgag tag) ybxH-X1:gtctcgagTATACACTGGGTGTCAATG
Reverse Primer:(gtggatcc tag) ybxH-BA:gtggatccCGCTTCGTTCAATTGCTCC
Downstream:
Forward Primer:(gtaagctt tag) ybxH-HD:gtaagcttGAATATCCCGATCATGATTT
Reverse Primer:(gtctcgag tag) ybxH-X2: gtctcgagACAAACTCCGCAATCCACC
Linearization:
Length of the Deletion: 22 to 127 Length: 106


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YBXHdd