Data for the Constructed Mutant: YYDJd

General Information

Name of the MutantYYDJd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size720 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYDJd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yydJ-F: aagaagcttGAGTATTAGCAACAAAGTTATC
Reverse Primer:(ggaggatcc tag) yydJ-R: ggaggatccAGTATAGATGTTCTCTCC
Length of the Cloned Region: 21 to 273 Length: 253
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYDJd