Data for the Constructed Mutant: YYDGd

General Information

Name of the MutantYYDGd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size957 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YYDGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yydG-F: aagaagcttATCAATTTAGATTCAAGGTG
Reverse Primer:(ggaggatcc tag) yydg-R: ggaggatccTATAGTCAGTGCTATCAC
Length of the Cloned Region: 22 to 342 Length: 321
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYDGd