Data for the Constructed Mutant: FBPd

General Information

Name of the MutantFBPd (disruption)
Used VectorpMutinT3
Used Enzyme SitesBglII
Gene Size2013 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for FBPd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) yydE-F2: cgaggcagatctATTCACCTTATCATGCAC
Reverse Primer:(cgaggcagatctgaattc tag) yydE-R: cgaggcagatctgaattcCGTTTAACGCTTCTTTCG
Length of the Cloned Region: 29 to 409 Length: 381
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of FBPd