Data for the Constructed Mutant: YYDAd

General Information

Name of the MutantYYDAd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size477 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYDAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yydA-F: aagaagcttATCAATATTGTGACAATC
Reverse Primer:(ggaggatcc tag) yydA-R: ggaggatccGGATAGGATGCGGTC
Length of the Cloned Region: 7 to 195 Length: 189
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYDAd